Plasmid_Backbone

Part:BBa_K371051:Design

Designed by: Ruijun Zhu   Group: iGEM10_USTC   (2010-10-27)


pSB1C5 (1bp mutation of pSB1C3 to remove SacI)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2051
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2051
    Illegal XhoI site found at 1034
    Illegal XhoI site found at 1927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2051
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2051
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2066
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

In order to make our working plasmid compatible to BBF RFC 53, we search the restriction enzymen site in pSB1C3 plasmid. And we found a SacI site in the pSB1C3. The site is between downstream of chloramphenicol and the T0 terminator. We think the mutation does not affect the regular function of pSB1C3 plasmid. So all of our following experiment of fusion protein was done in this new plasmid.


Source

this part comes from the parts.igem BBa_pSB1C3

References